partial purification of a potent immunosuppressive factor excreted from leishmania major promastigote and amastigote

نویسندگان

محسن ابوالحسنی

mohsen abolhassani هایده دارابی

haideh darabi

چکیده

recent scientific evidence indicates that distinct patterns of susceptibility in balb/c mice to leishmania major infection are attributable to the differential expansion of distinct cd4+ t-cell subsets and their cytokines production. production of the th1 cytokine ifn-g is associated with resistance, whereas production of the th2 cytokines il-4 and il-10 are associated with extreme susceptibility. the major host immune defense mechanism against leishmania is activation of macrophages by inf-γ derived from t cells. the inability of susceptible hosts to mount the immune response necessary to activate macrophage and destroy the parasites may be due to the parasite-specific proteins that are able to suppress the immune system. in the present study, we have semi-purified the excreted antigens of leishmania major promastigote and amastigote by column chromatography. the isolated fraction showed a potent immunosuppressive activity on normal balb/c mice lymphocytes stimulated with mitogens. fifteen microgram of the isolated fraction caused 81% suppression of lymphocyte proliferation. these data may suggest that the parasite by secreting immunosuppressive factor down regulate the immune system and as a result survive in the body

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عنوان ژورنال:
iranian biomedical journal

جلد ۸، شماره ۲، صفحات ۹۵-۹۹

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